Molecular Testing of Serial Blood Specimens from Patients with Early Lyme Disease during Treatment Reveals Changing Coinfection with Mixtures of Borrelia burgdorferi Genotypes

Published Date
Antimicrobial Agents and Chemotherapy
Antimicrob Agents Chemother. 2019; 63 (7)
Mosel MR
Carolan HE
Rebman AW
Castro S
Massire C
Ecker DJ
Soloski MJ
Aucott JN
Eshoo MW

Borrelia burgdorferi is the etiological agent of Lyme disease. In the current study, we used direct-detection PCR and electrospray ionization mass spectrometry to monitor and genotype B. burgdorferi isolates from serially collected whole-blood specimens from patients clinically diagnosed with early Lyme disease before and during 21 days of antibiotic therapy. B. burgdorferi isolates were detected up to 3 weeks after the initiation of antibiotic treatment, with ratios of coinfecting B. burgdorferi genotypes changing over time.