Evaluation of Borrelia Real Time PCR DNA Targeting OspA, FlaB and 5S-23S IGS and Borrelia 16S rRNA RT-qPCR

Published Date
Journal
Journal of Microbiological Methods
Citation
J Microbiol Methods. 2014 Dec;107:41-6
DOI
10.1016/j.mimet.2014.09.001
Authors
de Leeuw BHCGM
Maraha B
Hollemans L
Sprong H
Brandenburg AH
Westenend PJ
Kusters JG
Abstract

Borrelia burgdorferi non-sensu lato (s.l.) strains occurred in the Netherlands. A multiplex OspA, FlaB, IGS real time PCR was compared to 16S rRNA/rDNA RT-qPCR with lower average Cycle threshold (Ct) and LOD on strain dilutions. Multiplexing increased sensitivity on CSF samples (n=74), distinguishing B. burgdorferi s.l. from non-s.l. strains.